학술논문
Effect of Trehalose on Cryopreservation of Hariana Bull Spermatozoa.
Document Type
Article
Author
Source
Subject
*TREHALOSE
*SPERMATOZOA
*GLUTATHIONE reductase
*BULLS
*SPERM motility
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Language
ISSN
2394-0247
Abstract
The present experiment was designed to study the effect of supplementation of Trehalose on freezability and antioxidant activity of Hariana bull semen. Semen samples were diluted in TFYG extender containing different concentrations of Trehalose (10 mM, 30 mM & 50 mM). The control samples were extended with TFYG alone. Results clearly indicated that, 30 mM Trehalose group had significantly (P<0.05) higher percentage of individual sperm motility, sperm viability, and intact acrosome in comparison to the control and other Trehalose supplemented groups. In biochemical assays, no clear-cut demarcation for antioxidant enzyme was found for Trehalose supplementation. However, Trehalose supplementation has showed increase activity for Glutathione reductase (GR). The results obtained clearly indicated that supplementation of Trehalose (30 mM) to extender prior to cryopreservation improves Hariana bull sperm quality. [ABSTRACT FROM AUTHOR]