부산대학교 도서관

Objectives Transmitted drug resistance ( TDR) can impair the response to first-line antiretroviral therapy. In treatment-naïve patients chronically infected with HIV type 1 ( HIV-1), it was previously shown through Sanger sequencing that TDR was more common in men who have sex with men ( MSM) than in other transmission risk groups. We aimed to compare two HIV-1 transmission groups in terms of the presence of TDR mutations. Methods We investigated, through Sanger sequencing and ultradeep sequencing ( UDS), the presence of resistance mutations, both in majority (> 20%) and in minority (1−20%) proportions, in 70 treatment-naïve MSM and 70 treatment-naïve heterosexual patients who recently screened positive for HIV-1. Results The global prevalence of TDR was not significantly different between the two groups, either by Sanger or by UDS. Nevertheless, a higher frequency of nucleoside reverse transcriptase inhibitor TDR was observed among heterosexual patients ( P = 0.04). There was also a trend for a higher frequency of TDR among MSM infected with HIV-1 subtype B compared with MSM infected with HIV-1 non-B subtypes ( P = 0.06). Conclusions Ultradeep sequencing UDS allowed sensitive monitoring of TDR, and highlighted some disparities between transmission groups. [ABSTRACT FROM AUTHOR]