학술논문
Human Seminal Plasma Fosters CD4+ Regulatory T-cell Phenotype and Transforming Growth Factor-β1 Expression.
Document Type
Article
Source
Subject
*SEMINAL proteins
*T cells
*PHENOTYPES
*TRANSFORMING growth factors
*GENE expression
*FEMALE reproductive organs
*LABORATORY mice
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Language
ISSN
1046-7408
Abstract
Problem Semen mediates expansion of CD4+ regulatory T cells ( Treg) in the murine female reproductive tract. The impact of semen on Treg in humans, however, remains unclear. Method of Study Using seminal plasma ( SP) from 20 healthy donors, we investigated the impact of human semen on CD4+ T-cell expression of CD127 and CD49d as well as CD4+ CD127low CD49dlow Treg proliferation, apoptosis, and intracellular expression of Fox P3, TGF-β1, and IL-10. Results SP reduced CD4+ T-cell expression of CD127 and CD49d and increased the proportion of CD127low CD49dlow Treg. This increase was non-proliferative, mediated in part via the conversion of CD4+ helper T cells into Fox P3− but not Fox P3+ Treg. Additionally, SP induced an increase in intracellular expression of the immunosuppressive cytokine TGF-β1 by the Fox P3− but not Fox P3+ Treg. SP had no impact on Treg intracellular expression of IL-10. Conclusion Human seminal plasma fosters the non-proliferative increase in the proportion and immunoregulatory activity of Fox P3− Treg. [ABSTRACT FROM AUTHOR]