학술논문
Multiple Nucleocapsid Structural Forms of Shrimp White Spot Syndrome Virus Suggests a Novel Viral Morphogenetic Pathway.
Document Type
Article
Author
Huang, Hui-Ju; Tang, Sen-Lin; Chang, Yuan-Chih; Wang, Hao-Ching; Ng, Tze Hann; Garmann, Rees F.; Chen, Yu-Wen; Huang, Jiun-Yan; Kumar, Ramya; Chang, Sheng-Hsiung; Wu, Shang-Rung; Chao, Chih-Yu; Matoba, Kyoko; Kenji, Iwasaki; Gelbart, William M.; Ko, Tzu-Ping; Wang, Hwei-Jiung; Lo, Chu-Fang; Chen, Li-Li; Wang, Han-Ching
Source
Subject
*WHITE spot syndrome virus
*WHITELEG shrimp
*SHRIMPS
*IMMUNOELECTRON microscopy
*VIRUS diseases
*CRYOGENIC grinding
*TRANSMISSION electron microscopy
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Language
ISSN
1661-6596
Abstract
White spot syndrome virus (WSSV) is a very large dsDNA virus. The accepted shape of the WSSV virion has been as ellipsoidal, with a tail-like extension. However, due to the scarcity of reliable references, the pathogenesis and morphogenesis of WSSV are not well understood. Here, we used transmission electron microscopy (TEM) and cryogenic electron microscopy (Cryo-EM) to address some knowledge gaps. We concluded that mature WSSV virions with a stout oval-like shape do not have tail-like extensions. Furthermore, there were two distinct ends in WSSV nucleocapsids: a portal cap and a closed base. A C14 symmetric structure of the WSSV nucleocapsid was also proposed, according to our Cryo-EM map. Immunoelectron microscopy (IEM) revealed that VP664 proteins, the main components of the 14 assembly units, form a ring-like architecture. Moreover, WSSV nucleocapsids were also observed to undergo unique helical dissociation. Based on these new results, we propose a novel morphogenetic pathway of WSSV. [ABSTRACT FROM AUTHOR]