학술논문
Detecting Cytosolic Peptide Delivery with the GFP Complementation Assay in the Low Micromolar Range.
Document Type
Article
Author
Source
Subject
*PEPTIDES
*GENE transfection
*GREEN fluorescent protein
*PLASMIDS
*ELECTROPORATION
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Language
ISSN
0044-8249
Abstract
Transfection of cells with a plasmid encoding for the first ten strands of the GFP protein (GFP1-10) provides the means to detect cytosolic peptide import at low micromolar concentrations. Cytosolic import of the eleventh strand of the GFP protein either by electroporation or by cell-penetrating peptide-mediated import leads to formation of the full-length GFP protein and fluorescence. An increase in sensitivity is achieved through structural modifications of the peptide and the expression of GFP1-10 as a fusion protein with mCherry. [ABSTRACT FROM AUTHOR]