학술논문
27P Artemisia absinthium nanoparticles induce apoptosis in breast cancer cells via inhibiting vesicular trafficking related proteins.
Document Type
Article
Author
Source
Subject
*CELL cycle proteins
*CANCER cells
*BREAST cancer
*CELL cycle
*PROTEOMICS
*ARTEMISIA
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Language
ISSN
0923-7534
Abstract
Background The limitations of the currently available anti-breast cancer agents like they exert side effects can be overcome by combining two approaches together i.e. loading of herbal drugs into the nanoparticles. Methods Keeping in mind the tumor micro environment, double triggered (temperature and pH responsive) polymeric nanoparticles (NPs) were prepared and characterized by TEM, DLS and IR. The cytotoxicity of the different part extracts of A. absinthium was evaluated on the breast cancer cell lines (MCF-7 & MDA MB-231) by MTT and LDH assay. The extract with least IC50 value (whole plant extract) was loaded into the NPs and various cytotoxic parameters like MTT assay, CFSE cell proliferation assay, apoptosis assay, cell cycle study and DAPI nuclear staining was performed to evaluate cytotoxicity against breast cancer cell lines by. To further delineate the mechanism of action of extract loaded NPs, nano-LC-MS/MS analysis was performed. Finally, the probable mechanism behind the caused cytotoxicity was explored by the interactome analysis of highly differentially expressed proteins via STRING online tool. Results The cytotoxic studies together with proteome analysis suggest that extract loaded NPs effectively inhibits cell proliferation and induce apoptosis and cell cycle arrest in G0/G1 phase in both the breast cancer cell lines via significantly modulating the expression of the key proteins related to the proliferation, vesicular trafficking, apoptosis, and tumor suppression. Moreover, interactome analysis uncovered the key apoptotic proteins responsible for the caused cytotoxicity. Conclusions In conclusion, encapsulation enhances the cytotoxicity of the whole extract of A. absinthium and causes apoptosis in breast cancer cells. Furthermore, nano LCMS/MS and STRING analysis uncover the probable mechanism behind the caused cytotoxicity. Legal entity responsible for the study Saima Wajid. Funding Has not received any funding. Disclosure All authors have declared no conflicts of interest. [ABSTRACT FROM AUTHOR]