부산대학교 도서관

The microbiome of the mosquito Aedes aegypti is largely determined by the environment and influences mosquito susceptibility for arthropod-borne viruses (arboviruses). Larval interactions with different bacteria can have carry-over effects on adult Ae. aegypti replication of arboviruses, but little is known about the role that mosquito host genetics play in determining how larval-bacterial interactions shape Ae aegypti susceptibility to arboviruses. To address this question, we isolated single bacterial isolates and complex microbiomes from Ae. aegypti larvae from various field sites in Senegal. Either single bacterial isolates or complex microbiomes were added to two different genetic backgrounds of Ae. aegypti in a gnotobiotic larval system. Using 16S amplicon sequencing we showed that the bacterial community structure differs between the two genotypes of Ae. aegypti when given identical microbiomes, and the abundance of single bacterial taxa differed between Ae. aegypti genotypes. Using single bacterial isolates or the entire preserved complex microbiome, we tested the ability of specific larval microbiomes to drive differences in infection rates for Zika virus in different genetic backgrounds of Ae. aegypti. We observed that the proportion of Zika virus-infected adults was dependent on the interaction between the larval microbiome and Ae. aegypti host genetics. By using the larval microbiome as a component of the environment, these results demonstrate that interactions between the Ae. aegypti genotype and its environment can influence Zika virus infection. As Ae. aegypti expands and adapts to new environments under climate change, an understanding of how different genotypes interact with the same environment will be crucial for implementing arbovirus transmission control strategies. Author summary: Adult mosquitoes transmit many viruses, including Zika virus, during the process of taking a bloodmeal from human hosts. An important parameter of how well a mosquito is at transmitting viruses is whether the mosquito can become infected and replicate the virus. Different mosquito populations can be genetically distinct from each other, and in some cases genetic differences are associated with the habitat they live in. An important factor of the mosquito habitat is the water source in which larvae develop into adults. These water sources harbor diverse bacterial communities and are the source of the larval microbiome, which is known to influence how well the mosquito can become infected with viruses as an adult. Here we show that the effect of the larval microbiome on adult susceptibility to Zika virus depends on the genotype of the mosquito. These results indicate that different genetic backgrounds of mosquitoes interact with their habitat differently and this has important consequences for how easy it is for a mosquito to become infected. [ABSTRACT FROM AUTHOR]