학술논문
Glucuronan lyases from family PL7 use a Tyr/Tyr syn β-elimination catalytic mechanism for glucuronan breakdown.
Document Type
Article
Author
Source
Subject
*LYASES
*ACID catalysts
*CRYSTAL structure
*ALGINIC acid
*HISTIDINE
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Language
ISSN
1359-7345
Abstract
TpPL7A and TpPL7B, members of CAZy family PL7, act as β-glucuronan lyases. TpPL7A diverges by lacking the catalytic histidine, identified as the Brønsted base in PL7 alginate lyases. Our research, including TpPL7A's crystal structure, and mutagenesis studies, reveals a shared syn-β-elimination mechanism with a single tyrosine serving as both base and acid catalyst. This mechanism may extend to subfamily PL7_4 glucuronan lyases. [ABSTRACT FROM AUTHOR]