학술논문
Fluorescence In Situ Hybridization (FISH) for the Characterization and Monitoring of Primary Cultures from Human Tumors.
Document Type
Article
Author
Román-Lladó, Ruth; Aguado, Cristina; Jordana-Ariza, Núria; Roca-Arias, Jaume; Rodríguez, Sonia; Aldeguer, Erika; Garzón-Ibañez, Mónica; García-Peláez, Beatriz; Vives-Usano, Marta; Giménez-Capitán, Ana; Aguilar, Andrés; Martinez-Bueno, Alejandro; Gonzalez Cao, María; García-Casabal, Florencia; Viteri, Santiago; Mayo de las Casas, Clara; Rosell, Rafael; Angel Molina-Vila, Miguel
Source
Subject
*FLUORESCENCE in situ hybridization
*CANCER cell culture
*CELL populations
*DNA copy number variations
*ASCITES
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Language
ISSN
2673-5261
Abstract
Genetic and drug sensitivity assays on primary cultures are not only of basic but also of translational interest and could eventually aid oncologists in the selection of treatments. However, cancer cells need to be identified and differentiated from the non-tumor cells always present in primary cultures. Also, successive passages can change the proportions of these two subpopulations. In this study, we propose fluorescence in situ hybridization (FISH) analysis on cell smears to determine the presence of tumor cells in primary cultures obtained from patients carrying translocations or copy number gains. FISH proved to be an easy, fast, economic, and reliable method of characterizing cell populations, which could be used repeatedly at different passages to monitor variations and to confirm the maintenance of translocations and copy number gains throughout the culture process. [ABSTRACT FROM AUTHOR]