학술논문
An integrated platform for in vitro single-site cell electroporation: Controlled delivery and electrodes functionalization
Document Type
Article
Author
Source
Subject
*ELECTROPORATION
*CANCER cells
*TITANIUM dioxide
*NANOSTRUCTURED materials
*MICROFLUIDIC devices
*GOLD electrodes
*GREEN fluorescent protein
*CELL adhesion
*
*
*
*
*
*
*
Language
ISSN
0925-4005
Abstract
Abstract: This work presents an improved platform for single-site electroporation and controlled transfectants delivery. The device consists of a gold microelectrode array (MEA) with integrated microfluidics and nanostructured titanium dioxide (ns-TiO2) functionalized electrodes for the improvement of cell adhesion. Human cervical cancer cells (HeLa) have been successfully cultivated on chip surface using traditional protocols. The system has been previously tested by electroporating HeLa cells with Lucifer Yellow (LY) and then, in order to validate the approach and cell viability, with plasmid for the enhanced expression of Green Fluorescence Protein (pEGFP-N1) delivered through microchannels. [Copyright &y& Elsevier]