학술논문
3′-Modified Oligo(2′- O -Methylribonucleotides) as Improved Probes for Hybridization with RNA.
Document Type
Article
Source
Subject
*RNA
*NUCLEIC acid hybridization
*OLIGONUCLEOTIDES
*NUCLEOSIDES
*OLIGOMERS
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*
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Language
ISSN
1525-7770
Abstract
A series of octa(2′- O -methylribonucleotides) with an additional 3′-terminal deoxynucleoside (T, dC, dA or dG) linked by the 3′-3′ (“inverted”) bond was synthesized. The exceptional stability of these oligomers to a 3′-exonuclease (SVP) and nucleases in culture medium containing 10% heat-inactivated fetal calf serum was demonstrated. It was shown that the addition of the 3′-dangling inverted deoxynucleoside increases substantially the thermal stability of the duplexes of oligo(2′- O -methylribonucleotides) with complementary RNA and DNA in the case of a relatively weak terminal A m U(T) pair and enhances the mismatch sensitivity. [ABSTRACT FROM AUTHOR]