학술논문
Resource plasticity-driven carbon-nitrogen budgeting enables specialization and division of labor in a clonal community.
Document Type
Article
Source
Subject
*DIVISION of labor
*NUCLEOTIDE synthesis
*TREHALOSE
*SACCHAROMYCES cerevisiae
*ASPARTIC acid
*COMMUNITIES
*
*
*
*
*
Language
ISSN
2050-084X
Abstract
Previously, we found that in glucose-limited Saccharomyces cerevisiae colonies, metabolic constraints drive cells into groups exhibiting gluconeogenic or glycolytic states. In that study, threshold amounts of trehalose - a limiting, produced carbon-resource, controls the emergence and self-organization of cells exhibiting the glycolytic state, serving as a carbon source that fuels glycolysis (Varahan et al., 2019). We now discover that the plasticity of use of a nonlimiting resource, aspartate, controls both resource production and the emergence of heterogeneous cell states, based on differential metabolic budgeting. In gluconeogenic cells, aspartate is a carbon source for trehalose production, while in glycolytic cells using trehalose for carbon, aspartate is predominantly a nitrogen source for nucleotide synthesis. This metabolic plasticity of aspartate enables carbon-nitrogen budgeting, thereby driving the biochemical selforganization of distinct cell states. Through this organization, cells in each state exhibit true division of labor, providing growth/survival advantages for the whole community. [ABSTRACT FROM AUTHOR]