부산대학교 도서관

Using monospecific reagents from invertebrates, particularly those directed against terminal N-acetyl-neuraminyl residues, it is deduced that Burkitt EB2 lymphoma cells have terminal N-acetyl-neuraminyl groups linked glycosidically to D-galactose units. Evidence was obtained with agglutinins derived from plants that terminal D-galactosyl and inner-chain N-acetyl-glucosaminyl groups are also components of heterosacchande chains in the cell membrane. Neither terminal nor penultimate N-acetyl-D-galactosaminyl groups were detected by N-acetyl-D-galactosaminyl specific snail agglutinins, nor were myxovirus receptors present to any significant extent, thus differentiating Burkitt EB2 lymphoma cells from erythrocytes. The T- and Forssman antigens were absent. Burkitt EB2 lymphoma cells actively regenerate neuraminyl end-groups following treatment with neuraminidase; this biosynthesis is suppressed by sub-lethal irradiation. Several other heterophile lectin receptors were detected in Burkitt EB2 lymphoma cells during our investigations, and it was confirmed that Phaseolus vulgaris (PHA) extract has separate erythrocyte-agglutinating and mitogenic properties. All studies were carried out on intact living Burkitt EB2 lyrnphoma cells. [ABSTRACT FROM AUTHOR]