학술논문
Eicosapentaenoic acid (EPA)-induced inhibitory effects on porcine coronary and cerebral arteries involve inhibition of prostanoid TP receptors.
Document Type
Article
Author
Yoshioka, Kento; Obara, Keisuke; Oikawa, Shunya; Uemura, Kohei; Yamaguchi, Akina; Fujisawa, Kazuki; Hanazawa, Hitomi; Fujiwara, Miki; Endoh, Taison; Suzuki, Taichi; De Dios Regadera, Montserrat; Ito, Daichi; Saitoh, Noboru; Nakagome, Yutaka; Yamashita, Toma; Kiguchi, Mayu; Saito, Yuka; Nakao, Yuri; Miyaji, Hinako; Ou, Guanghan
Source
Subject
*CORONARY arteries
*EICOSAPENTAENOIC acid
*CEREBRAL arteries
*BLOOD vessels
*BASILAR artery
*PROSTAGLANDINS
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Language
ISSN
2045-2322
Abstract
This study was performed to elucidate whether eicosapentaenoic acid (EPA) suppresses spasm-prone blood vessel contractions induced by a thromboxane mimetic (U46619) and prostaglandin F2α (PGF2α) and determine whether the primary target of EPA is the prostanoid TP receptor. Accordingly, we assessed: (1) the tension changes in porcine basilar and coronary arteries, and (2) changes in the Fura-2 (an intracellular Ca2+ indicator) fluorescence intensity ratio at 510 nm elicited by 340/380 nm excitation (F340/380) in 293T cells expressing the human TP receptor (TP-293T cells) and those expressing the human prostanoid FP receptor (FP-293T cells). EPA inhibited both porcine basilar and coronary artery contractions induced by U46619 and PGF2α in a concentration-dependent manner, but it did not affect the contractions induced by 80 mM KCl. EPA also inhibited the increase in F340/380 induced by U46619 and PGF2α in TP-293T cells. In contrast, EPA showed only a marginal effect on the increase in F340/380 induced by PGF2α in FP-293T cells. These findings indicate that EPA strongly suppresses the porcine basilar and coronary artery contractions mediated by TP receptor and that inhibition of TP receptors partly underlies the EPA-induced inhibitory effects on these arterial contractions. [ABSTRACT FROM AUTHOR]