학술논문
SLC23A3 is a renal hypoxanthine transporter.
Document Type
Article
Author
Source
Subject
*HYPOXANTHINE
*XANTHINE
*XENOPUS
*OVUM
*KIDNEYS
*SODIUM channels
*
*
*
*
*
Language
ISSN
1525-7770
Abstract
LLC-PK1 renal cells show Na+-dependent and Na+-independent hypoxanthine uptake. While the latter is inhibited by adenine, neither are inhibited by xanthine. In rats, intestinal Na+-dependent hypoxanthine transporter Slc23a4 is not expressed in the kidney, and its action is inhibited by xanthine. This study aimed to clone Slc23a4-paralog SLC23A3 from the human kidney and investigate its hypoxanthine transport activity. We observed Na+-dependent 10 nM [3H]-hypoxanthine uptake in SLC23A3 RNA-injected Xenopus oocytes. Moreover, 100 μM xanthine did not inhibit Na+-independent 300 nM [3H]-hypoxanthine uptake, whereas 100 μM adenine did. These results confirm that SLC23A3 is a hypoxanthine transporter in the human kidney. [ABSTRACT FROM AUTHOR]