학술논문
Expression Analysis of the Circular RNA Molecules in the Human Retinal Cells Treated with Homocysteine.
Document Type
Article
Author
Source
Subject
*CIRCULAR RNA
*RNA analysis
*HOMOCYSTEINE
*RHODOPSIN
*MOLECULES
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Language
ISSN
0271-3683
Abstract
Purpose: To characterize the global profile of circular RNAs (circRNAs) and their differential expression levels in homocysteine (Hcy)-treated ARPE-19 cells, a line of human retinal pigment epithelial (RPE) cells. Materials and Methods: We treated ARPE-19 cells with and without Hcy to investigate the influence of Hcy on circRNA expression levels using dedicated human circRNA microarrays. Results: A total of 12,233 circRNAs were identified out of them 54 were differentially expressed (17 were down-regulated, and 37 were up-regulated) with a fold change >2.0 (p < 0.05) in Hcy-treated versus untreated cells. Conclusions: To our knowledge, this is the first report profiling circRNAs in human RPE cells post-Hcy treatment mimicking hyperhomocysteinemic (HHcy) conditions that negatively affect retinal biology and vision. These findings are of potential clinical significance as they will help understand Hcy metabolism and HHcy-mediated diseases and identify potential diagnostic and therapeutic targets for eye diseases that are caused by elevated Hcy concentrations. [ABSTRACT FROM AUTHOR]