학술논문
Survival of human cadaver skin on severe combined immune deficiency pigs: Proof of concept.
Document Type
Article
Author
Source
Subject
*ANIMAL experimentation
*AUTOGRAFTS
*CRYOPRESERVATION of organs, tissues, etc.
*DEAD
*GRAFT rejection
*GRAFT versus host reaction
*INFLAMMATION
*SCARS
*SKIN grafting
*SWINE
*T cells
*WOUND healing
*XENOGRAFTS
*SEVERE combined immunodeficiency
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Language
ISSN
1067-1927
Abstract
Transplantation of human xenografts onto immunocompromised mice is a powerful research tool for studying wound healing. However, differences in healing between humans and mice and their small size limit this model. We determined whether human cadaver skin xenografts transplanted onto pigs with severe combined immune deficiency (SCID) would survive and not be rejected. Meshed (1:1.5), cryopreserved human cadaver skin was transplanted onto 10 partial thickness dermatome wounds in each of two normal domestic pigs and two SCID pigs. Autografts (n = 2/animal) from the four animals were used as controls. In normal pigs, all autografts were engrafted and healed with a minimal, if any, inflammation and scarring. All human xenografts were rejected by the normal pigs within 5–11 days and associated with an intense T‐cell inflammatory response. In contrast, both autografts and xenografts were engrafted and survived the 28‐day study in the SCID pigs with a minimal inflammation and no gross scarring. [ABSTRACT FROM AUTHOR]