학술논문
A multistep computational approach reveals a neuro-mesenchymal cell population in the embryonic hematopoietic stem cell niche.
Document Type
Article
Author
Miladinovic, Olivera; Canto, Pierre-Yves; Pouget, Claire; Piau, Olivier; Radic, Nevenka; Freschu, Priscilla; Megherbi, Alexandre; Prats, Carla Brujas; Jacques, Sebastien; Hirsinger, Estelle; Geeverding, Audrey; Dufour, Sylvie; Petit, Laurence; Souyri, Michele; North, Trista; Isambert, Hervé; Traver, David; Jaffredo, Thierry; Charbord, Pierre; Durand, Charles
Source
Subject
*STEM cell niches
*HEMATOPOIETIC stem cells
*EMBRYONIC stem cells
*CELL populations
*EXTRACELLULAR matrix
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Language
ISSN
0950-1991
Abstract
The first hematopoietic stem and progenitor cells (HSPCs) emerge in the Aorta-Gonad-Mesonephros (AGM) region of the mid-gestation mouse embryo. However, the precise nature of their supportive mesenchymal microenvironment remains largely unexplored. Here, we profiled transcriptomes of laser micro-dissected aortic tissues at three developmental stages and individual AGM cells. Computational analyses allowed the identification of several cell subpopulations within the E11.5 AGM mesenchyme, with the presence of a yet unidentified subpopulation characterized by the dual expression of genes implicated in adhesive or neuronal functions.We confirmed the identity of this cell subset as a neuro-mesenchymal population, through morphological and lineage tracing assays. Loss of function in the zebrafish confirmed that Decorin, a characteristic extracellular matrix component of the neuro-mesenchyme, is essential for HSPC development. We further demonstrated that this cell population is not merely derived from the neural crest, and hence, is a bona fide novel subpopulation of the AGM mesenchyme. [ABSTRACT FROM AUTHOR]