부산대학교 도서관

Aims: In the present study, we analysed the diversity of indigenous arbuscular mycorrhizal fungi (AMF) colonising both the roots and rhizosphere soil of an annual herbaceous species, Bromus rubens, and a perennial herbaceous species, Brachypodium retusum, co-occurring in the same Mediterranean, semiarid degraded area. The intention was to study whether these two species promoted the diversity of AM fungi in their rhizospheres differently and to ascertain whether the AMF community harboured by an annual plant species differed from that harboured by a perennial species when both grew in the same place. Methods: The AMF large subunit ribosomal RNA genes (LSU) were subjected to nested PCR, cloning, sequencing and phylogenetic analysis. Results: Twenty AMF sequence types belonging to Glomus group A, Glomus group B and Diversispora were identified. The two plant species differed in the AMF community composition in their roots, B. rubens showing a higher diversity of AMF than B. retusum. However the composition of the AMF communities associated with the two rhizosphere soils was similar. Conclusions: These results suggest that the management of these Mediterranean, semiarid degraded areas should include the promotion of annual herbaceous plant communities in order to maintain the sustainability and productivity of these ecosystems. [ABSTRACT FROM AUTHOR]