학술논문
Cell-cell adhesions in embryonic stem cells regulate the stability and transcriptional activity of β-catenin.
Document Type
Article
Author
Source
Subject
*EMBRYONIC stem cells
*WNT signal transduction
*RF values (Chromatography)
*CADHERINS
*CELL physiology
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Language
ISSN
0014-5793
Abstract
E-cadherin (CDH1) is involved in maintaining cell-cell adhesions in embryonic stem cells (ESCs). However, its function in the context of cell fate decisions is largely unknown. Using mouse ESCs (mESCs), we demonstrate that E-cadherin and b-catenin interact at the membrane and continue to do so upon internalization within the cell. Cdh1 -/- mESCs failed to form tight colonies, with altered differentiation, marker expression and retention of pluripotency factors during differentiation. Interestingly, Cdh1 -/- mESCs showed dramatically reduced b-catenin levels. Transcriptional profiling of Cdh1 -/- mESCs displayed a significant alteration in the expression of a subset of bcatenin targets in a cell state- and GSK3b-dependent manner. Our findings hint at hitherto unknown roles played by E-cadherin in regulating the activity of b-catenin in ESCs. [ABSTRACT FROM AUTHOR]