학술논문
Broccoli microgreens juice reduces body weight by enhancing insulin sensitivity and modulating gut microbiota in high-fat diet-induced C57BL/6J obese mice.
Document Type
Article
Author
Source
Subject
*REDUCING diets
*BROCCOLI
*BEVERAGES
*FAT content of food
*GUT microbiome
*ANIMAL experimentation
*DIETARY supplements
*OXIDATIVE stress
*WEIGHT loss
*DESCRIPTIVE statistics
*INSULIN resistance
*MICE
*ADIPOSE tissues
*SHORT-chain fatty acids
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Language
ISSN
1436-6207
Abstract
Purpose: This study aimed to explore the protective effect of broccoli microgreens juice (BMJ) during C57BL/6J mice obesity development. Methods: The obese model mice, induced by feeding high-fat diet (HFD), were treated with BMJ by gavage for 10 weeks. Melbine was gavaged at 300 mg/(kg bw)/d, as a positive control group. Results: BMJ supplementation significantly reduced white adipose tissues (WAT) mass, the body weight and adipocyte size, and increased water intake in HFD-fed mice. Moreover, it improved glucose tolerance, reduced insulin level and HOMA-IR value, and alleviated insulin resistance. Compared with the HFD group, BMJ supplementation significantly increased the relative abundance of Bacteroidetes and decreased the ratio of Firmicutes to Bacteroidetes at the phylum level, and enriched Bacteroides_acidifaciens at the species level. These changes in the composition of gut microbiota are associated with the production of short-chain fatty acids (SCFAs), and reduced LPS levels, and had an obvious anti-inflammatory effect. Conclusions: These findings suggested that the protective effects of BMJ on diet-induced obesity may be involved in gut microbiota–SCFAs–LPS–inflammatory axis. In addition, BMJ can enhance liver antioxidant capacity and reduce liver fat accumulation. Consequently, these results sustain BMJ as a novel functional food for obesity, on the basis of its opposing effects on HFD-induced obesity in mice. [ABSTRACT FROM AUTHOR]