학술논문
Identification and Expression Cloning of a Leptin Receptor, OB-R.
Document Type
Article
Author
Tartaglia, Louis A.; Dembski, Marlene; Xun Weng; Nanhua Deng; Janice Culpepper; Devos, Rene; Richards, Grayson J.; Campfield, L. Arthur; Clark, Frederick T.; Deeds, Jim; Muir, Craig; Sanker, Sean; Moriarty, Ann; Moore, Karen J.; Smutko, John S.; Mays, Gail G.; Woolf, Elizabeth A.; Monroe, Cheryl A.; Tepper, Robert I.
Source
Subject
*GENE mapping
*LEPTIN
*HORMONES
*GENETIC engineering
*BIOCHEMISTRY
*MAMMAL body composition
*MESSENGER RNA
*
*
*
*
*
*
Language
ISSN
0092-8674
Abstract
The ob gene product, leptin, is an important circulating signal for the regulation of body weight. To identify high affinity leptin-binding sites, we generated a series of leptin-alkaline phosphatase (AP) fusion proteins as well as [125]leptin. After a binding survey of cell lines and tissues, we identified leptin-binding sites in the mouse choroid plexus. A cDNA expression library was prepared from mouse choroid plexus and screened with a leptin-AP fusion protein to identify a leptin receptor (OB-R). OB-R is a single membrane-spanning receptor most related to the gp 130 signal-transducing component of the IL-6 receptor, the G-CSF receptor, and the LIF receptor. OB-R mRNA is expressed not only in choroid plexus, but also in several other tissues, including hypothalamus. Genetic mapping of the gene encoding OB-R shows that it is within the 5.1 cM interval of mouse chromosome 4 that contains the db locus. [ABSTRACT FROM AUTHOR]