학술논문
Unveiling the complete genome sequence of clerodendrum chlorotic spot virus, a putative dichorhavirus infecting ornamental plants.
Document Type
Article
Author
Source
Subject
*CHLOROSIS
*BREVIPALPUS
*RHABDOVIRUSES
*REVERSE transcriptase polymerase chain reaction
*ORNAMENTAL plants
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Language
ISSN
0304-8608
Abstract
The genus Dichorhavirus includes plant-infecting rhabdoviruses with bisegmented genomes that are horizontally transmitted by false spider mites of the genus Brevipalpus. The complete genome sequences of three isolates of the putative dichorhavirus clerodendrum chlorotic spot virus were determined using next-generation sequencing (Illumina) and traditional RT-PCR. Their genome organization, sequence similarity and phylogenetic relationship to other viruses, and transmissibility by Brevipalpus yothersi mites support the assignment of these viruses to a new species of dichorhavirus, as suggested previously. New data are discussed stressing the reliability of the current rules for species demarcation and taxonomic status criteria within the genus Dichorhavirus. [ABSTRACT FROM AUTHOR]