학술논문
Computational identification of Penaeus monodon microRNA genes and their targets.
Document Type
Article
Source
Subject
*PENAEUS monodon
*MICRORNA
*NON-coding RNA
*ANIMAL genetics
*NUCLEOTIDE sequence
*GENE expression
*COMPUTATIONAL biology
*INVERTEBRATES
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Language
ISSN
0125-3395
Abstract
MicroRNAs (miRNAs) are a distinct class of small non-coding RNAs, ~22 nt long, found in a wide variety of organisms. They play important regulatory roles by silencing gene activities at the post-transcriptional level. In this work, we developed a computational workflow to identify conserved miRNA genes in the 10,536 unique Penaeus monodon expressed sequence tags (ESTs). After removing all simple repeats and coding regions in the ESTs, the workflow uses both the conservation of miRNA sequences and several filters obtained from pre-miRNA secondary structure properties to identify conserved miRNAs. Finally, we discovered six potential conserved miRNA genes such as mir-4152, mir-466k, miR-32*, lin-4, mir-1346 and mir-4310. [ABSTRACT FROM AUTHOR]