학술논문
Rapid development of neutralizing and diagnostic SARS-COV-2 mouse monoclonal antibodies.
Document Type
Article
Author
Chapman, Asheley P.; Tang, Xiaoling; Lee, Joo R.; Chida, Asiya; Mercer, Kristina; Wharton, Rebekah E.; Kainulainen, Markus; Harcourt, Jennifer L.; Martines, Roosecelis B.; Schroeder, Michelle; Zhao, Liangjun; Bryksin, Anton; Zhou, Bin; Bergeron, Eric; Bollweg, Brigid C.; Tamin, Azaibi; Thornburg, Natalie; Wentworth, David E.; Petway, David; Bagarozzi, Dennis A.
Source
Subject
*COVID-19 pandemic
*MONOCLONAL antibodies
*AMINO acid receptors
*ANGIOTENSIN converting enzyme
*HYBRIDOMAS
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Language
ISSN
2045-2322
Abstract
The need for high-affinity, SARS-CoV-2-specific monoclonal antibodies (mAbs) is critical in the face of the global COVID-19 pandemic, as such reagents can have important diagnostic, research, and therapeutic applications. Of greatest interest is the ~ 300 amino acid receptor binding domain (RBD) within the S1 subunit of the spike protein because of its key interaction with the human angiotensin converting enzyme 2 (hACE2) receptor present on many cell types, especially lung epithelial cells. We report here the development and functional characterization of 29 nM-affinity mouse SARS-CoV-2 mAbs created by an accelerated immunization and hybridoma screening process. Differing functions, including binding of diverse protein epitopes, viral neutralization, impact on RBD-hACE2 binding, and immunohistochemical staining of infected lung tissue, were correlated with variable gene usage and sequence. [ABSTRACT FROM AUTHOR]