학술논문

A PCR-free technology to detect and quantify microRNAs directly from human plasma.
Document Type
Article
Source
Analyst. 12/7/2018, Vol. 143 Issue 23, p5676-5682. 7p.
Subject
*MICRORNA
*PLASMA gases
*POLYMERASE chain reaction
Language
ISSN
0003-2654
Abstract
A novel sensitive, specific and rapid method for the detection and quantification of microRNAs without requiring extraction from their biological sources is now available using a novel chemical based, PCR-free technology for nucleic acid testing. In this study, we both demonstrate how this method can be used to profile miR-451a, an important miRNA in erythropoiesis, and compare with the gold standard RT-qPCR. [ABSTRACT FROM AUTHOR]