학술논문
Comparison of the Hemagglutination Inhibition Test and IgG ELISA in Categorizing Primary and Secondary Dengue Infections Based on the Plaque Reduction Neutralization Test.
Document Type
Article
Author
Source
Subject
*DIAGNOSIS of fever
*IMMUNOGLOBULIN analysis
*MIXED infections
*BIOLOGICAL assay
*COMPARATIVE studies
*DENGUE
*ENZYME-linked immunosorbent assay
*HEMAGGLUTINATION tests
*PROBABILITY theory
*REGRESSION analysis
*DATA analysis
*DATA analysis software
*DESCRIPTIVE statistics
*NEUTRALIZATION tests
*DIAGNOSIS
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Language
ISSN
2314-6133
Abstract
Secondary dengue infection by heterotypic serotypes is associated with severe manifestations of disease, that is, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The World Health Organization (WHO) has recommended criteria based on the hemagglutination inhibition (HI) test to distinguish between primary and secondary dengue infections. Since the HI test has practical limitations and disadvantages, we evaluated the accuracy of WHO HI criteria and compared it with criteria based on an IgG enzyme-linked immunosorbent assay (ELISA) using a plaque reduction neutralization test (PRNT) as the gold standard. Both WHO HI criteria and IgG ELISA criteria performed strongly (16/16) in determining primary infection. However, to determine secondary infection, the IgG ELISA criteria performed better (72/73) compared to the WHO HI criteria (23/73). [ABSTRACT FROM AUTHOR]