부산대학교 도서관

Dinucleotides (NpN′; N and N′ are A, U, G, or C, n = 2-7) are naturally occurring physiologically active compounds. Despite the interest in dinucleotides, the composition of their complexes with metal ions as well as their conformations and species distribution in living systems are understudied. Therefore, we investigated a series of Mg and Ca complexes of NpN′s. Potentiometric titrations indicated that a longer dinucleotide polyphosphate (N is A or G, n = 3-5) linker yields more stable complexes (e.g., log K of 2.70, 3.27, and 3.73 for ApA-Mg, n = 3, 4, 5, respectively). The base (A or G) or ion (Mg or Ca) has a minor effect on $$ \log K^{\text{M}}{}_{\text{ML}} $$ values. In a physiological medium, the longer ApAs ( n = 4, 5) are predicted to occur mostly as the Mg/Ca complexes. P NMR monitored titrations of NpN′s with Mg/Ca ions showed that the middle phosphates of the dinucleotides coordinate with Mg/Ca. Multidimensional potential of mean force (PMF) molecular dynamics (MD) simulations suggest that ApA and ApA coordinate Mg and Ca ions in both inner-sphere and outer-sphere modes. The PMF MD simulations additionally provide a detailed picture of the possible coordination sites, as well as the cation binding process. Moreover, both NMR and MD simulations showed that the conformation of the nucleoside moieties in NpN′-Mg/Ca complexes remains the same as that of free mononucleotides. Graphical abstract: Dinucleotides form complexes with Mg/Ca ions which coordinate to the middle phosphate groups in the polyphosphate chain. Mg/Ca coordination slightly increases intramolecular π-stacking interactions in the complexes, whereas the conformation of nucleoside moieties remains almost the same as in the corresponding Na salts. Considering physiological Mg/Ca concentrations, the ApA-Mg/ApA-Mg and ApA-Ca/ApA-Ca species are expected to be dominant (approximately 70-90 %) in living systems versus the free dinucleotide.[Figure not available: see fulltext.] [ABSTRACT FROM AUTHOR]