학술논문
Finger stick blood test to assess postvaccination SARS‐CoV‐2 neutralizing antibody response against variants.
Document Type
Article
Author
Lim, Sing Mei; Cheng, Hoi Lok; Jia, Huan; Kongsuphol, Patthara; D/O Shunmuganathan, Bhuvaneshwari; Chen, Ming Wei; Ng, Say Yong; Gao, Xiaohong; Turaga, Shuvan Prashant; Heussler, Sascha P.; Somani, Jyoti; Sengupta, Sharmila; Tay, Dousabel M. Y.; McBee, Megan E.; Young, Barnaby E.; MacAry, Paul A.; Sikes, Hadley D.; Preiser, Peter R.
Source
Subject
*SARS-CoV-2 Delta variant
*ANTIBODY formation
*SARS-CoV-2
*BLOOD testing
*FINGERS
*LANDSCAPE changes
*IMMUNOGLOBULINS
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Language
ISSN
2380-6761
Abstract
There is clinical need for a quantifiable point‐of‐care (PoC) SARS‐CoV‐2 neutralizing antibody (nAb) test that is adaptable with the pandemic's changing landscape. Here, we present a rapid and semi‐quantitative nAb test that uses finger stick or venous blood to assess the nAb response of vaccinated population against wild‐type (WT), alpha, beta, gamma, and delta variant RBDs. It captures a clinically relevant range of nAb levels, and effectively differentiates prevaccination, post first dose, and post second dose vaccination samples within 10 min. The data observed against alpha, beta, gamma, and delta variants agrees with published results evaluated in established serology tests. Finally, our test revealed a substantial reduction in nAb level for beta, gamma, and delta variants between early BNT162b2 vaccination group (within 3 months) and later vaccination group (post 3 months). This test is highly suited for PoC settings and provides an insightful nAb response in a postvaccinated population. [ABSTRACT FROM AUTHOR]