학술논문
Expression and enzyme activity of cytochrome P450 enzymes CYP3A4 and CYP3A5 in human skin and tissue‐engineered skin equivalents.
Document Type
Article
Author
Source
Subject
*XENOBIOTIC biodegradation
*SKIN tests
*CYTOCHROME P-450 regulation
*MASS spectrometry
*BIOTRANSFORMATION (Metabolism)
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Language
ISSN
0906-6705
Abstract
Abstract: CYP3A4 and CYP4A5 share specificity for a wide range of xenobiotics with the CYP3 subfamily collectively involved in the biotransformation of approximately 30% of all drugs. CYP3A4/5 mRNA transcripts have been reported in the skin, yet knowledge of their protein expression and function is lacking. In this study, we observed gene and protein expression of CYP3A4/5 in both human skin and tissue‐engineered skin equivalents (TESEs), and enzyme activity was detected using the model substrate benzyl‐O‐methyl‐cyanocoumarin. Mass spectrometric analysis of TESE lysates following testosterone application revealed a time‐dependent increase in metabolite production, confirming the functional expression of these enzymes in skin. [ABSTRACT FROM AUTHOR]