학술논문
Brother of the regulator of the imprinted site (BORIS) variant subfamily 6 is a novel target of lung cancer stem-like cell immunotherapy.
Document Type
Article
Author
Horibe, Ryota; Hirohashi, Yoshihiko; Asano, Takuya; Mariya, Tasuku; Suzuki, Takeshi; Takaya, Akari; Saijo, Hiroshi; Shionoya, Yosuke; Kubo, Terufumi; Nakatsugawa, Munehide; Kanaseki, Takayuki; Tsukahara, Tomohide; Watanabe, Kazue; Atsuyama, Eri; Toji, Shingo; Hirano, Hiroshi; Hasegawa, Tadashi; Takahashi, Hiroki; Sato, Noriyuki; Torigoe, Toshihiko
Source
Subject
*LUNG cancer treatment
*STEM cell treatment
*CANCER immunotherapy
*TARGETED drug delivery
*CANCER-related mortality
*DRUG resistance in cancer cells
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Language
ISSN
1932-6203
Abstract
Lung cancer is one of the most common malignancies with a high rate of mortality. Lung cancer stem-like cells (CSCs)/ cancer-initiating cells (CICs) play major role in resistance to treatments, recurrence and distant metastasis and eradication of CSCs/CICs is crucial to improve recent therapy. Cytotoxic T lymphocytes (CTLs) are major effectors of cancer immunotherapy, and CTLs recognize antigenic peptides derived from antigens that are presented by major histocompatibility complex (MHC) class I molecules. In this study, we analyzed the potency of a cancer-testis (CT) antigen, brother of the regulator of the imprinted site variant subfamily 6 (BORIS sf6), in lung CSC/CIC immunotherapy. BORIS sf6 mRNA was expressed in lung carcinoma cells (9/19), especially in sphere-cultured lung cancer stem-like cells, and in primary lung carcinoma tissues (4/9) by RT-PCR. Immunohistochemical staining using BORIS sf6-specific antibody revealed that high expression of BORIS sf6 is related to poorer prognosis. CTLs could be induced by using a human leukocyte antigen, (HLA)-A2 restricted antigenic peptide (BORIS C34_24(9)), from all of 3 HLA-A2-positive individuals, and CTL clone cells specific for BORIS C34_24(9) peptide could recognize BORIS sf6-positive, HLA-A2-positive lung carcinoma cells. These results indicate that BORIS sf6 is a novel target of lung cancer immunotherapy that might be useful for targeting treatment-resistant lung cancer stem-like cells. [ABSTRACT FROM AUTHOR]