부산대학교 도서관

A new strain that produced a lipase capable of enantioselectively hydrolyzing the ethyl ester of (R)-flurbiprofen was isolated from soil samples and identified as Bacillus cereus C71. The optimal temperature and pH for the hydrolysis reaction with resting cells were 35°C and 8.0, respectively. The surfactant Tween-40 enhanced the reaction rate and enantioselectivity. A small-scale production of (R)-flurbiprofen was conducted with 10 mmol L-1 substrate solution under the optimum conditions. After 36 h of reaction, 50% of the initial flurbiprofen ethyl ester was hydrolyzed to (R)-flurbiprofen with an enantiomeric excess (ee p) of 96%, and an enantiomeric ratio (E) of >100. The cells could be reused and retained 60% of initial activity after recycling for six times. [ABSTRACT FROM AUTHOR]