부산대학교 도서관

This study summarizes the findings of our research on the genesis of methane, its content and distribution in permafrost horizons of different age and origin. Supported by reliable data from a broad geographical sweep, these findings confirm the presence of methane in permanently frozen fine-grained sediments. In contrast to the omnipresence of carbon dioxide in permafrost, methane-containing horizons (up to 40.0 mL kg−1) alternate with strata free of methane. Discrete methane-containing horizons representing over tens of thousands of years are indicative of the absence of methane diffusion through the frozen layers. Along with the isotopic composition of CH4 carbon (δ13C −64‰ to −99‰), this confirms its biological origin and points to in situ formation of this biogenic gas. Using 14C-labeled substrates, the possibility of methane formation within permafrost was experimentally shown, as confirmed by δ13C values. Extremely low values (near −99‰) indicate that the process of CH4 formation is accompanied by the substantial fractionation of carbon isotopes. For the first time, cultures of methane-forming archaea, Methanosarcina mazei strain JL01 VKM B-2370, Methanobacterium sp. strain M2 VKM B-2371 and Methanobacterium sp. strain MK4 VKM B-2440 from permafrost, were isolated and described. [ABSTRACT FROM AUTHOR]