학술논문
Novel Assay to Measure Seroprevalence of Zika Virus in the Philippines.
Document Type
Journal Article
Author
Source
Subject
*ZIKA virus
*SEROPREVALENCE
*DENGUE viruses
*FLAVIVIRUSES
*SEROTYPES
*ANTIGEN-antibody reactions
*RESEARCH
*DENGUE
*RESEARCH methodology
*MEDICAL cooperation
*EVALUATION research
*COMPARATIVE studies
*RESEARCH funding
*VIRAL antibodies
*EPIDEMIOLOGICAL research
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Language
ISSN
1080-6040
Abstract
Zika virus (ZIKV) is a member of the Flaviviridae family, which includes other clinically notable viruses such as the 4 dengue virus serotypes (DENV-1-4). Distinguishing DENVs from ZIKV using the established serologic assays widely used for monitoring DENV transmission is difficult because of antibody cross-reactivity between these closely related flaviviruses. We describe a modified and improved recombinant envelope domain III-based serologic assay for detecting ZIKV type-specific antibodies in regions with endemic DENV transmission. When the assay was used to measure ZIKV seroprevalence in 2017 among children 9-14 years of age living in a region of the Philippines with endemic DENV transmission, we observed a ZIKV seroprevalence of 18%. Investigators should consider using the ZIKV envelope domain III-based assay, which is simple and readily adaptable for use in standard clinical and public health laboratories, to assess ZIKV seroprevalence in areas with endemic DENV transmission. [ABSTRACT FROM AUTHOR]