학술논문
Golgi Cisternal Unstacking Stimulates COPI Vesicle Budding and Protein Transport.
Document Type
Article
Source
Subject
*GOLGI apparatus
*MAMMAL genetics
*PLANT propagation
*PROTEIN analysis
*MITOSIS
*CELL cycle
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Language
ISSN
1932-6203
Abstract
The Golgi apparatus in mammalian cells is composed of flattened cisternae that are densely packed to form stacks. We have used the Golgi stacking protein GRASP65 as a tool to modify the stacking state of Golgi cisternae. We established an assay to measure protein transport to the cell surface in post-mitotic cells in which the Golgi was unstacked. Cells with an unstacked Golgi showed a higher transport rate compared to cells with stacked Golgi membranes. Vesicle budding from unstacked cisternae in vitro was significantly increased compared to stacked membranes. These results suggest that Golgi cisternal stacking can directly regulate vesicle formation and thus the rate of protein transport through the Golgi. The results further suggest that at the onset of mitosis, unstacking of cisternae allows extensive and rapid vesiculation of the Golgi in preparation for its subsequent partitioning. [ABSTRACT FROM AUTHOR]