학술논문
Next-Generation Sequencing of Coccidioides immitis Isolated during Cluster Investigation.
Document Type
Article
Author
Engelthaler, David M.; Chiller, Tom; Schupp, James A.; Colvin, Joshua; Beckstrom-Sternberg, Stephen M.; Driebe, Elizabeth M.; Moses, Tracy; Tembe, Waibhav; Sinari, Shripad; Beckstrom-Sternberg, James S.; Christoforides, Alexis; Pearson, John V.; Carpten, John; Keim, Paul; Peterson, Ashley; Terashita, Dawn; Balajee, S. Arunmozhi
Source
Subject
*NUCLEOTIDE sequence
*COCCIDIOIDES immitis
*TRANSPLANTATION of organs, tissues, etc.
*COCCIDIOIDOMYCOSIS
*GENETIC polymorphisms
*EPIDEMIOLOGY
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Language
ISSN
1080-6040
Abstract
Next-generation sequencing enables use of whole-genome sequence typing (WGST) as a viable and discriminatory tool for genotyping and molecular epidemiologic analysis. We used WGST to confirm the linkage of a cluster of Coccidioides immitis isolates from 3 patients who received organ transplants from a single donor who later had postive test results for coccidioidomycosis. Isolates from the 3 patients were nearly genetically identical (a total of 3 single nucleotide polymorphisms identified among them), thereby demonstrating direct descent of the 3 isolates from an original isolate. We used WGST to demonstrate the genotypic relatedness of C. immitis isolates that were also epidemio-logically linked. Thus, WGST offers unique benefits to public health for investigation of clusters considered to be linked to a single source. [ABSTRACT FROM AUTHOR]