학술논문
Identification of potential biomarkers for post-traumatic complications released after trauma–hemorrhage from murine Kupffer cells and its investigation in lung and liver.
Document Type
Article
Author
Source
Subject
*BIOMARKERS
*HEMORRHAGE
*KUPFFER cells
*GENE expression
*WOUNDS & injuries
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Language
ISSN
1354-750X
Abstract
Context:Early diagnosis of complications after severe trauma by specific biomarkers remains difficult. Objective:Identify potential new biomarkers for early diagnosis of post-traumatic complications. Material and methods:Mice underwent pressure-controlled hemorrhage or sham procedure. Four hours later, genome-wide expression of isolated Kupffer cells was compared with controls using Affymetrix-Genechip-Expression-Analysis and real-time-PCR. Results:Expression analysis and real-time-PCR revealed a significant increase of gene expression ofCxcl10, Il4ra, Csf2rb2, Lcn2,andGbp5. Conclusion:Cxcl10, Il4ra, Csf2rb2, Lcn2,andGbp5might represent new biomarkers for early diagnosis of post-traumatic complications, if they are linked to the development of post-traumatic complications. [ABSTRACT FROM PUBLISHER]