부산대학교 도서관

Studies in humans and cell culture as well as bioinformatics suggested that Coenzyme Q_{10} (CoQ_{10}) functions as an anti-inflammatory molecule. Here we studied the influence of CoQ_{10} (Kaneka Q_{10}™ on secretion of the pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-α) by using the human and murine monocytic cell lines THP-1 and RAW264.7 expressing human apolipoprotein E3 (apoE3) or pro-inflammatory apoE4. Incubation of cells with physiological (0.1–10 μM) and supra-physiological (> 10 to < 100 μM) concentrations of CoQ_{10} led to an intracellular accumulation of its reduced form without any cytotoxic effects. Stimulation of cell models with lipopolysaccharide (LPS) resulted in a substantially release of TNF-α. When THP-1 cells were pre-incubated with 10 μM CoQ_{10}, the LPS-induced TNF-α release was significantly decreased to 72 ± 32%. This effect is similar to those obtained by 10 μM N-Acetyl-Cysteine, a well known reference antioxidant. In RAW264.7-apoE3 and -apoE4 cells, significant reductions of LPS-induced TNF-α secretion to 73.3 ± 2.8% and 74.7 ± 8.9% were found with 2.5 μM and 75 μM CoQ_{10}, respectively. In conclusion, CoQ_{10} has moderate anti-inflammatory effects in two monocytic cell lines which could be mediated by its antioxidant activity. [ABSTRACT FROM AUTHOR]