학술논문
The age and genomic integrity of neurons after cortical stroke in humans.
Document Type
Article
Author
Huttner, Hagen B; Bergmann, Olaf; Salehpour, Mehran; Rácz, Attila; Tatarishvili, Jemal; Lindgren, Emma; Csonka, Tamás; Csiba, László; Hortobágyi, Tibor; Méhes, Gábor; Englund, Elisabet; Solnestam, Beata Werne; Zdunek, Sofia; Scharenberg, Christian; Ström, Lena; Ståhl, Patrik; Sigurgeirsson, Benjamin; Dahl, Andreas; Schwab, Stefan; Possnert, Göran
Source
Subject
*NUCLEIC acids
*GENES
*NEURONS
*GENOMES
*CEREBRAL cortex
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Language
ISSN
1097-6256
Abstract
It has been unclear whether ischemic stroke induces neurogenesis or neuronal DNA rearrangements in the human neocortex. Using immunohistochemistry; transcriptome, genome and ploidy analyses; and determination of nuclear bomb test-derived 14C concentration in neuronal DNA, we found neither to be the case. A large proportion of cortical neurons displayed DNA fragmentation and DNA repair a short time after stroke, whereas neurons at chronic stages after stroke showed DNA integrity, demonstrating the relevance of an intact genome for survival. [ABSTRACT FROM AUTHOR]