학술논문
Quantification method of osmotin-like proteins in sweet pepper and eggplant by liquid chromatography/tandem mass spectrometry based on the aqua technology.
Document Type
Article
Source
Subject
*EGGPLANT
*SWEET peppers
*LIQUID chromatography-mass spectrometry
*ALLERGENS
*PEPTIDES
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Language
ISSN
1094-2912
Abstract
PR5-like protein and osmotin-like protein PR-5 are osmotin-like proteins found in sweet pepper and eggplant, respectively. These belong to the family of 5 pathogenesis-related proteins, which are considered a plentiful source of allergens. Recently, we established an assay to quantify protein NP24, an osmotin-like protein of tomato, using absolute quantification method with a stable isotope-labeled internal standard peptide (GQTWVINAPR[13C6,15N4]) and liquid chromatography/tandem mass spectrometry. In this study, the peaks of GQTWVINAPR derived from PR5-like protein and osmotin-like protein PR-5 were detected by multiple reaction monitoring. The results indicated that the assay could be applied for the quantification of these proteins. [ABSTRACT FROM AUTHOR]