부산대학교 도서관

Abstract Coenzyme Q 10 (CoQ 10) is a redox molecule critical for the proper function of energy metabolism and antioxidant defenses. Despite its essential role in cellular metabolism, the regulation of CoQ 10 biosynthesis in humans remains mostly unknown. Herein, we determined that PPTC7 is a regulatory protein of CoQ 10 biosynthesis required for human cell survival. We demonstrated by in vitro approaches that PPTC7 is a bona fide protein phosphatase that dephosphorylates the human COQ7. Expression modulation experiments determined that human PPTC7 dictates cellular CoQ 10 content. Using two different approaches (PPTC7 over-expression and caloric restriction), we demonstrated that PPTC7 facilitates and improves the human cell adaptation to respiratory conditions. Moreover, we determined that the physiological role of PPTC7 takes place in the adaptation to starvation and pro-oxidant conditions, facilitating the induction of mitochondrial metabolism while preventing the accumulation of ROS. Here we unveil the first post-translational mechanism regulating CoQ 10 biosynthesis in humans and propose targeting the induction of PPTC7 activity/expression for the treatment of CoQ 10 -related mitochondrial diseases. Graphical abstract Unlabelled Image Highlights • The human gene PPTC7 encodes for a mitochondrial phosphatase of the PPM family. • COQ7, a protein involved in CoQ 10 biosynthesis, is target in vitro of PPTC7. • PPTC7 regulates CoQ 10 biosynthesis in human cells. • Decreased levels of glucose increase PPTC7 expression in HeLa cells. • PPTC7 overexpression improves mitochondrial metabolism. [ABSTRACT FROM AUTHOR]