학술논문
Sialic acid dependence in rotavirus host cell invasion.
Document Type
Article
Author
Source
Subject
*NUCLEAR magnetic resonance spectroscopy
*NEURAMINIDASE
*ROTAVIRUSES
*SIALIC acids
*CELLULAR recognition
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Language
ISSN
1552-4450
Abstract
We used NMR spectroscopy, molecular modeling and infectivity competition assays to investigate the key interactions between the spike protein (VP8*) from 'sialidase-insensitive' human Wa and 'sialidase-sensitive' porcine CRW-8 rotaviruses and the glycans of gangliosides GM1 and GD1a. Our data provide strong evidence that N-acetylneuraminic acid is a key determinant for binding of these rotaviruses. This is in contrast to the widely accepted paradigm that sialic acids are irrelevant in host cell recognition by sialidase-insensitive rotaviruses. [ABSTRACT FROM AUTHOR]