학술논문
Tentative function and structure prediction of putative genes in the whole genome of ligninolytic Brevibacillus agri DSM6348 for the better elucidation of cellular metabolism.
Document Type
Article
Source
Subject
*MOLECULAR motor proteins
*MYXOCOCCUS xanthus
*CYTOSKELETAL proteins
*GENES
*PROTEIN domains
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Language
ISSN
0094-243X
Abstract
This study involves the prediction of the structure and function of putative genes in the genome of pathogenic and ligninolytic bacteria Brevibacillus agri, for better elucidation of its cellular metabolism. A total number of 50 hypothetical genes were collected from the genome of the bacteria B. agri. Bioinformatics online tools were used in this study to analyze the protein sequence and domains. The tools used include KEGG, PDB, SMART, ScanProsite, InterPro, ProtParam, NCBI-BLAST, HHpred, CPH(Copenhagen) modeling, and PSIpred. Out of 50 Hypothetical genes 4 genes were predicted. The function was analyzed for BA6348_03080, BA6348_03335, BA6348_03780, BA6348_01995 and they showed E-values of 7e-9,0, 0058, 6.5e-15, 0.000081 respectively. Based on the bioinformatics online tools tentative function and structure were predicted for BA6348_03080, BA6348_03335, BA6348_03780, BA6348_01995, and the functions were found to be similar to antimicrobial activity, isomerase, structural and motor proteins respectively, and the validation was done for the gene BA6348_01995 which showed similarity to motor protein from gram-negative bacteria Myxococcus xanthus. [ABSTRACT FROM AUTHOR]