학술논문
New long noncoding RNA biomarkers and ceRNA networks on miR-616-3p in colorectal cancer: Bioinformatics-based study.
Document Type
Article
Author
Abdolvand, Mohammad; Chermahini, Zahra Mohammadi; Bahaloo, Sahar; Emami, Mohammad Hassan; Fahim, Alireza; Rahimi, Hojjatolah; Amjadi, Elham; Maghool, Fatemeh; Rohani, Fattah; Dadkhah, Mina; Farhadian, Nooshin; Vatandoust, Nasimeh; Abdolvand, Shirin; Darehsari, Maliheh Roozbahani; Chehelgerdi, Mohammad; Beni, Faeze Ahmadi; Khodadoostan, Mahsa; Hemati, Simin; Salehi, Mansoor
Source
Subject
*RNA metabolism
*STATISTICAL correlation
*COMPETITIVE endogenous RNA
*RESEARCH funding
*MICRORNA
*POLYMERASE chain reaction
*COLORECTAL cancer
*TUMOR markers
*DESCRIPTIVE statistics
*GENE expression
*KAPLAN-Meier estimator
*LOG-rank test
*BIOINFORMATICS
*MOLECULAR structure
*CASE-control method
*RESEARCH
*DATA analysis software
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Language
ISSN
1735-1995
Abstract
Background: Cancer development is aided by the role of long noncoding RNAs (lncRNAs) that act as competing endogenous RNAs (ceRNAs) absorbing microRNAs (miRNAs). We aimed to discover a novel regulatory axis in colorectal cancer (CRC) and potential biomarkers based on miR-616-3p. Materials and Methods: The gene expression omnibus database was mined for differentially expressed lncRNAs (DELs) and mRNAs. LncRNAs and mRNAs were predicted using the RegRNA and TargetScan databases. A combination of the ciBioPortal and Ensemble databases was used to locate the mRNAs. Cytoscape 3.7.1-built CeRNA networks. A quantitative real-time polymerase chain reaction (qRT-PCR) was utilized to confirm the expression levels of these RNA molecules. Statistical analyses were implemented by GraphPad Prism 9. Results: qRT-PCR showed (Linc01282, lnc-MYADM-1:1, and Zinc Finger Protein 347 [ZNF347]) were overexpressed whereas, (salt-inducible kinases 1 [SIK1], and miR-616-3p) were down regulated. Conclusion: These results identify unique, unreported lncRNAs as CRC prognostic biomarkers, as well as prospective mRNAs as new treatment targets and predictive biomarkers for CRC. In addition, our study uncovered unexplored ceRNA networks that should be studied further in CRC. [ABSTRACT FROM AUTHOR]