학술논문
High-precision rapid testing of omicron SARS-CoV-2 variants in clinical samples using AI-nanopore.
Document Type
Article
Author
Murakami, Kaoru; Kubota, Shimpei I.; Tanaka, Kumiko; Tanaka, Hiroki; Akabane, Keiichiroh; Suzuki, Rigel; Shinohara, Yuta; Takei, Hiroyasu; Hashimoto, Shigeru; Tanaka, Yuki; Hojyo, Shintaro; Sakamoto, Osamu; Naono, Norihiko; Takaai, Takayui; Sato, Kazuki; Kojima, Yuichi; Harada, Toshiyuki; Hattori, Takeshi; Fuke, Satoshi; Yokota, Isao
Source
Subject
*SARS-CoV-2 Omicron variant
*SARS-CoV-2 Delta variant
*SARS-CoV-2
*COVID-19
*SENSITIVITY & specificity (Statistics)
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Language
ISSN
1473-0197
Abstract
A digital platform that can rapidly and accurately diagnose pathogenic viral variants, including SARS-CoV-2, will minimize pandemics, public anxiety, and economic losses. We recently reported an artificial intelligence (AI)-nanopore platform that enables testing for Wuhan SARS-CoV-2 with high sensitivity and specificity within five minutes. However, which parts of the virus are recognized by the platform are unknown. Similarly, whether the platform can detect SARS-CoV-2 variants or the presence of the virus in clinical samples needs further study. Here, we demonstrated the platform can distinguish SARS-CoV-2 variants. Further, it identified mutated Wuhan SARS-CoV-2 expressing spike proteins of the delta and omicron variants, indicating it discriminates spike proteins. Finally, we used the platform to identify omicron variants with a sensitivity and specificity of 100% and 94%, respectively, in saliva specimens from COVID-19 patients. Thus, our results demonstrate the AI-nanopore platform is an effective diagnostic tool for SARS-CoV-2 variants. [ABSTRACT FROM AUTHOR]