학술논문
Multispectral tracing in densely labeled mouse brain with nTracer.
Document Type
Article
Author
Roossien, Douglas H; Sadis, Benjamin V; Yan, Yan; Webb, John M; Min, Lia Y; Dizaji, Aslan S; Bogart, Luke J; Mazuski, Cristina; Huth, Robert S; Stecher, Johanna S; Akula, Sriakhila; Shen, Fred; Li, Ye; Xiao, Tingxin; Vandenbrink, Madeleine; Lichtman, Jeff W; Hensch, Takao K; Herzog, Erik D; Cai, Dawen
Source
Subject
*IMAGE processing software
*INTERNET servers
*CELL morphology
*BRAIN
*MICE
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Language
ISSN
1367-4803
Abstract
Summary This note describes nTracer, an ImageJ plug-in for user-guided, semi-automated tracing of multispectral fluorescent tissue samples. This approach allows for rapid and accurate reconstruction of whole cell morphology of large neuronal populations in densely labeled brains. Availability and implementation nTracer was written as a plug-in for the open source image processing software ImageJ. The software, instructional documentation, tutorial videos, sample image and sample tracing results are available at https://www.cai-lab.org/ntracer-tutorial. Supplementary information Supplementary data are available at Bioinformatics online. [ABSTRACT FROM AUTHOR]