학술논문

EXPRESSION PROFILING OF THE MANNURONAN C5-EPIMERASE MULTIGENIC FAMILY IN THE BROWN ALGA LAMINARIA DIGITATA (PHAEOPHYCEAE) UNDER BIOTIC STRESS CONDITIONS.
Document Type
Article
Source
Journal of Phycology. Oct2008, Vol. 44 Issue 5, p1250-1256. 7p. 2 Black and White Photographs, 1 Diagram, 1 Graph.
Subject
*ALGINATES
*MICROBIAL polysaccharides
*LAMINARIA digitata
*LAMINARIACEAE
*POLYMERASE chain reaction
*BROWN algae
*ALGAE
Language
ISSN
0022-3646
Abstract
The aim of this study was to profile expression of mannuronan C5-epimerase (ManC5-E) genes of Laminaria digitata (Huds.) J. V. Lamour. under treatment with brown alga cell wall components known to induce defense reaction in this organism. After determining that incubation in the presence of homo-guluronates increased the accumulation of ManC5-E transcripts, we cloned a number of partial ManC5-E genes from nonelicited and elicited specimens. In addition to these fragments, previously identified genes from sporophyte and protoplast cDNA libraries were considered for a clustering analysis. Several groups of ManC5-E genes were observed, including groups containing sequences specific from nonelicited and elicited sporophytes, and sequences isolated from protoplasts. Quantitative real-time PCR experiments were then carried out using genes representative of different groups. Results of transcript accumulation during protoplast regeneration and sporophyte elicitation supported the hypothesis that specific C5-epimerase genes are recruited under pathogen attack, enabling L. digitata to modify its cell wall in response to environmental stimuli. [ABSTRACT FROM AUTHOR]