부산대학교 도서관

The incorporation of [[sup 14]C]elaidic acid (trans18 : 1(n-9)) in phosphatidylcholine and phosphatidylethanolamine molecular species in isolated rat liver cells has been studied, and the results compared with the incorporation, previously published (B. Woldseth et al. Biochim Biophys Acta 1993;1167:296-302), of [[sup 14]C]palmitic acid (16 : 0) and [[sup 14]C]stearic acid (18 : 0) and with that of [[sup 14]C]oleic acid (cis18 : 1(n-9)). The pattern of incorporation in phospholipid molecular species is similar to that of [[sup 14]C]stearic acid and different from that of [[sup 14]C]palmitic acid. In phosphatidylcholine [[sup 14]C]trans18 : 1-18 : 2 and [[sup 14]C]trans18 : 1-20 : 4 were the most abundant species, and in phosphatidylethanolamine [[sup 14]C]trans18 : 1-20 : 4 was the predominant species. With increasing concentration of [[sup 14]C]elaidic acid increasing amounts of [[sup 14]C]trans18 : 1-[[sup 14]C]trans18 : 1 were found. The total incorporation in phospholipids was less than that of [[sup 14]C]stearic acid, but more than that of [[sup 14]C]palmitic acid. The distribution in percent of [[sup 14]C]elaidic acid in phospholipid classes was 8.8% in phosphatidylinositol, 1.8% in phosphatidylserine, 59.1% in phosphatidylcholine and 30.3% in phosphatidylethanolamine with 0.1 & mmol l[sup -1] substrate concentration. More [[sup 14]C]elaidic acid than [[sup 14]C]palmitic acid or [[sup 14]C]stearic acid was oxidized. The incorporation in phospholipids of [[sup 14]C]elaidic acid was very different from that of [[sup 14]C]oleic acid. The main species with [[sup 14]C]oleic acid were 16 : 0-[[sup 14]C]cis18 : 1 in phosphatidylcholine, and [[sup 14]C]cis18 : 1-20 : 4 in phosphatidylethanolamine. In some experiments [[sup 14]C]18 : 2(n-6) was incubated together with unlabelled elaidic or unlabelled trans-vaccenic acid (trans18 : 1(n-7)). In these experiments, more trans18 : 1-18 : 2 was formed from elaidic acid than from trans-vaccenic acid, especially in phosphatidylethanolamine. [ABSTRACT FROM AUTHOR]