학술논문
The Outer Chloroplast Envelope Protein OEP16-1 for Plastid Import of NADPH:Protochlorophyllide Oxidoreductase A in Arabidopsis thaliana.
Document Type
Article
Author
Source
Subject
*ARABIDOPSIS thaliana
*CHLOROPLASTS
*NAD (Coenzyme)
*PLANT enzymes
*PROTOCHLOROPHYLLIDE
*OXIDOREDUCTASES
*PLANT mutation
*REACTIVE oxygen species
*EFFECT of oxygen on plants
*PLANT genetics
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Language
ISSN
0032-0781
Abstract
The outer plastid envelope protein OEP16-1 was previously identified as an amino acid-selective channel protein and translocation pore for NADPH:protochlorophyllide oxidoreductase A (PORA). Reverse genetic approaches used to dissect these mutually not exclusive functions of OEP16-1 in planta have led to descriptions of different phenotypes resulting from the presence of several mutant lines in the SALK_024018 seed stock. In addition to the T-DNA insertion in the AtOEP16-1 gene, lines were purified that contain two additional T-DNA insertions and as yet unidentified point mutations. In a first attempt to resolve the genetic basis of four different lines in the SALK_024018 seed stock, we used genetic transformation with the OEP16-1 cDNA and segregation analyses after crossing out presumed point mutations. We show that AtOEP16-1 is involved in PORA precursor import and by virtue of this activity confers photoprotection onto etiolated seedlings during greening. [ABSTRACT FROM AUTHOR]