부산대학교 도서관

In this study, hetero-exopolysaccharide of Lactobacillus gasseri FR4 isolated from native chicken was produced and purified. The molecular weight of Lg EPS was found to be 1.86 × 10 5 Da. The gas chromatographic analysis revealed that the Lg EPS was majorly composed of glucose (65.31%), mannose (16.51%), galactose (8.45%), rhamnose (6.55%) and a small fraction of fucose (3.18%). The functional groups of Lg EPS were confirmed by FT-IR analysis. The 1D ( 1 H and 13 C) and 2D NMR (COSY and HSQC) analysis showed the presence of 1,6 linked-α- d -Glc p , 1,4 linked-α- d -Gal p , 1,3,4 linked-α- d -Man p , 1,3 linked-α- l - R ha p , 1,4 linked-α- l -Fuc p , 1,4 linked-β- d -Glc p , and β- d -Gal p -1 residues. SEM and AFM micrographs revealed the fibrous and porous nature of Lg EPS. Moreover, Lg EPS exhibited in vitro antioxidant, antibacterial and antibiofilm activity against various food borne pathogens, which proved that this Lg EPS might be used in food industries as an antioxidant agent, viscosifying agent and antimicrobial agent etc. [ABSTRACT FROM AUTHOR]